tatpipТехника и технология пищевых производствFood Processing: Techniques and Technology2074-94142313-1748Кемеровский государственный университет10.21603/2074-9414-2024-1-2486LMLTQJtatpip-158Research ArticleСтатьиГидролиз и окисление трудноразлагаемых субстратов микробными изолятами термальных источниковHydrolysis and Oxidation of Recalcitrant Substrates by Microbial Isolates from Hot Springshttps://orcid.org/0000-0002-8764-4049ДмитриеваА. И.DmitrievaA. I.

Кемерово

Elizaveta R. Faskhutdinova

Kemerovo

https://orcid.org/0000-0001-9711-2145ФасхутдиноваЕ. Р.FaskhutdinovaE. R.

Кемерово

Elizaveta R. Faskhutdinova

Kemerovo

faskhutdinovae.98@mail.ruhttps://orcid.org/0000-0002-3801-0023ЛаричевТ. А.LarichevT. A.

Кемерово

https://orcid.org/0000-0002-9061-1256ВеличковичН. С.

Кемерово

БогерВ. Ю.

Кемерово

АксеноваЛ. М.

Москва

Кемеровский государственный университетРоссияKemerovo State UniversityRussian FederationКемеровский государственный университетRussian FederationВсероссийский научно-исследовательский институт кондитерской промышленностиRussian Federation2024210820265412736Copyright © Дмитриева А.И., Фасхутдинова Е.Р., Ларичев Т.А., Величкович Н.С., Богер В.Ю., Аксенова Л.М., 20262026Дмитриева А.И., Фасхутдинова Е.Р., Ларичев Т.А., Величкович Н.С., Богер В.Ю., Аксенова Л.М.Dmitrieva A.I., Faskhutdinova E.R., Larichev T.A., Величкович Н.С., Богер В.Ю., Аксенова Л.М.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.fptt.ru/jour/article/view/158

Традиционные источники энергии загрязняют окружающую среду. Для снижения экологической нагрузки предлагается использовать альтернативный источник энергии на основе микроорганизмов – микробные топливные элементы. Еще одним полезным применением микробного топливного элемента является очистка сточных вод от трудноразлагаемых отходов. Целью исследования являлось изучение ферментативной способности изолятов термального источника Абаканский Аржан.

Объектами исследования служили изоляты родов Geobacter, Thermomonas и Rhodopseudomonas. Исследование кератинолитической активности осуществляли по ГОСТ Р 55987-2014. Определение хитинолитической активности проводили путем посева бактериальной суспензии уколом на чашки Петри со средой, содержащей хитин. Оценку липолитической активности осуществляли выращиванием изолятов в бульоне Штерна. Изучение способности изолятов к гидролизу ксилана проводили путем анализа скорости образования восстанавливающих сахаров. Целлюлазную активность изолятов измеряли по стандартной методике IUPAC. Каталазную активность оценивали на средах, содержащих 1 % бензин. Активность определяли газометрическим способом. Оптимальные параметры культивирования консорциумов определяли по количеству генерируемого напряжения.

Максимальной кератинолитической активностью обладал изолят Geobacter. Однако для изолята Thermomonas отмечена максимальная степень гидролиза белка – 80,1 ± 1,5 %. Изоляты Geobacter и Rhodopseudomonas проявляли большую литическую активность в отношении хитина – зоны лизиса превышали 3 мм. Изолят Geobacter достигал 350 ед. ксиланазной активности и 365 ед. целлюлазной активности; Thermomonas – 350 ед. ксиланазной активности и 360 ед. целлюлазной активности; Rhodopseudomonas – 310 ед. ксиланазной активности и 304 ед. целлюлазной активности. Максимальной каталазной активностью отличаются изоляты Geobacter и Thermomonas – 1,40 и 1,38 ед. активности соответственно. Максимальное генерирование энергии консорциумами изолятов осуществлялось при pH 8, температуре 45 °C и продолжительности культивирования 48 ч.

Изоляты Geobacter, Thermomonas и Rhodopseudomonas, выделенные из термального источника Абаканский Аржан, способны удалять трудноразлагаемые компоненты. Это делает перспективным их применение в биологической очистке сточных вод.

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The authors declare that there are no conflicts of interest present.